Slocombe S P, Piffanelli P, Fairbairn D, Bowra S, Hatzopoulos P, Tsiantis M, Murphy D J
Brassica and Oilseeds Research Department, Cambridge Laboratory, John Innes Centre, Norwich, United Kingdom.
Plant Physiol. 1994 Apr;104(4):1167-76. doi: 10.1104/pp.104.4.1167.
The nucleotide sequence of a Brassica napus stearoyl-acyl carrier protein desaturase gene (Bn10) is presented. This gene is one member of a family of four closely related genes expressed in oilseed rape. The expression of the promoter of this gene in transgenic tobacco was found to be temporally regulated in the developing seed tissues. However, the promoter was also particularly active in other oleogenic tissues such as the tapetum and pollen grains. This raises the interesting question of whether seed-expressed lipid synthesis genes are regulated by separate tissue-specific determinants or by a single factor common to all oleogenic tissues. Parts of the plants undergoing rapid development such as the components of immature flowers and seedlings also exhibited high levels of promoter activity. These tissues are likely to have an elevated requirement for membrane lipid synthesis. Stearoyl-acyl carrier protein desaturase transcript levels have previously been shown to be temporally regulated in the B. napus embryo (S.P. Slocombe, I. Cummins, R.P. Jarvis, D.J. Murphy [1992] Plant Mol Biol 20: 151-155). Evidence is presented demonstrating the induction of desaturase mRNA by abscisic acid in the embryo.
本文展示了甘蓝型油菜硬脂酰-酰基载体蛋白去饱和酶基因（Bn10）的核苷酸序列。该基因是油菜中表达的四个紧密相关基因家族的成员之一。研究发现，该基因启动子在转基因烟草发育中的种子组织中受到时间调控。然而，该启动子在其他产油组织如绒毡层和花粉粒中也特别活跃。这就引出了一个有趣的问题：种子中表达的脂质合成基因是受单独的组织特异性决定因素调控，还是受所有产油组织共有的单一因子调控。植物中快速发育的部分，如未成熟花和幼苗的组成部分，也表现出高水平的启动子活性。这些组织可能对膜脂合成有更高的需求。此前已表明，硬脂酰-酰基载体蛋白去饱和酶转录水平在甘蓝型油菜胚胎中受到时间调控（S.P. Slocombe、I. Cummins、R.P.Jarvis、D.J. Murphy [1992] Plant Mol Biol 20: 151 - 155）。本文提供的证据表明，胚胎中的去饱和酶mRNA受脱落酸诱导。
