Hillar M
Arch Int Physiol Biochim. 1978 Feb;86(1):11-24. doi: 10.3109/13813457809069880.
Glutamate dehydrogenase displays hyperchromicity at 256 nm and at 276 nm upon binding of diethylstilbestrol. Increase in absorbancy is linear at both regions up to 250 micrometer DES, and becomes parabolic at higher concentration of DES. ADP in the presence of DES causes decrease in absorbancy at 256 nm; absorbancy at 276 nm increased by DES is not affected by ADP. DES prevents spectral effects produced by GTP (decrease in absorbancy at 254 nm and at 276 nm). ADP still decreases absorbancy at 254 nm, leaving the 276 nm region unchanged. ADP enhances spectral effects produced by GTP. GTP, however, prevents changes produced by ADP.
己烯雌酚与谷氨酸脱氢酶结合后，在256纳米和276纳米处显示增色效应。在两个区域，吸光度的增加在高达250微摩尔己烯雌酚时呈线性，而在更高浓度的己烯雌酚下则变为抛物线形。在己烯雌酚存在的情况下，ADP会导致256纳米处的吸光度降低；己烯雌酚使276纳米处的吸光度增加不受ADP影响。己烯雌酚可防止GTP产生的光谱效应（254纳米和276纳米处吸光度降低）。ADP在254纳米处仍会降低吸光度，而276纳米区域保持不变。ADP增强了GTP产生的光谱效应。然而，GTP可防止ADP产生的变化。
