Hassan S M, Kerkhoff H, Troost D, Veldman H, Jennekens F G
Department of Neurology, Academic Hospital, Utrecht, The Netherlands.
Acta Neuropathol. 1994;87(4):405-10. doi: 10.1007/BF00313610.
We have used immunohistochemistry and electron microscopy to investigate the distribution of basic fibroblast growth factor (bFGF) in the peripheral motor system of the adult rat. In the lumbar segments of the spinal cord, bFGF immunoreactivity (bFGF-I) was seen in motor neurons and glial cells but not in axons. The neuronal immunostaining was seen as two or three intensely fluorescent spots in the nuclei with weak and more diffuse staining of the perinuclear cytoplasm. In the sciatic nerve, bFGF-I was seen in Schwann cells with strong intensity at the nodes of Ranvier. Axonal immunostaining could not be detected. At the electron microscopic level, the intense nodal immunostaining of Schwann cells was confirmed and was found to be localized to the Schwann cell membrane at the nodal gap. The intensity of staining decreased with distance from the node. In the soleus and gastrocnemius muscles, bFGF-I was seen at the motor endplates in sites corresponding to the motor nerve terminals in addition to faint staining within the muscle fibers. Electron microscopy showed that bFGF-I was localized within the nerve terminals. Histochemical localization of bFGF in the peripheral motor system is compatible with the functions ascribed for this protein in this system.
我们运用免疫组织化学和电子显微镜技术来研究碱性成纤维细胞生长因子（bFGF）在成年大鼠外周运动系统中的分布情况。在脊髓腰段，运动神经元和神经胶质细胞中可见bFGF免疫反应性（bFGF-I），但轴突中未见。神经元免疫染色表现为细胞核中有两三个强荧光斑点，核周细胞质染色较弱且更弥散。在坐骨神经中，施万细胞中有bFGF-I，在郎飞结处强度较强。未检测到轴突免疫染色。在电子显微镜水平，证实了施万细胞在结处的强烈免疫染色，且发现其定位于结间隙处的施万细胞膜。染色强度随离结的距离增加而降低。在比目鱼肌和腓肠肌中，除了肌纤维内有微弱染色外，在与运动神经末梢相对应的运动终板处可见bFGF-I。电子显微镜显示bFGF-I定位于神经末梢内。bFGF在周围运动系统中的组织化学定位与该蛋白在该系统中所具有的功能相符。
