Pharmacological effects of nerve growth factor and fibroblast growth factor applied to the transectioned sciatic nerve on neuron death in adult rat dorsal root ganglia.

Sciatic nerve transection performed on adult rats caused neuronal losses after 4 weeks in the L4-6 dorsal root ganglia (DRG) of 35% as compared to the unlesioned side. Nerve growth factor (NGF) administered at a single dose of 6000 Biological Units in silicone tubes fixed to the proximal nerve stump completely prevented these cell losses. Basic fibroblast growth factor (bFGF) also protected DRG neurons, but at the concentrations applied (6000 Trophic Units, tested on embryonic chick ciliary ganglion neurons), failed to maintain cell numbers identical to unoperated side. Our data indicate that NGF and bFGF protect adult sensory neurons from lesion-induced death. Preliminary results suggest that local accumulation of neurotrophic activities at the proximal nerve stump elicited by NGF and, possibly bFGF, might be involved in the beneficial effects of these proteins on the maintenance of axotomized sensory neurons.