Gumber S, Eamens G, Whittington R J
Faculty of Veterinary Science, The University of Sydney, Private Bag 3, Camden 2570, NSW, Australia.
Vet Microbiol. 2006 Jun 15;115(1-3):91-101. doi: 10.1016/j.vetmic.2006.01.003. Epub 2006 Feb 7.
The present study was designed to evaluate a commercial ELISA kit (Institut Pourquier) for the diagnosis of ovine and caprine paratuberculosis under Australian conditions and to compare its accuracy with the existing AGID test. The sensitivity of the ELISA in sheep and goats was 34.9% and 56.4%, with a specificity of 98.8% and 100.0%, respectively. Sensitivity of AGID was 13.8% for sheep and 39.5% for goats, with specificity of 100.0% for both species. The sensitivity of the ELISA in sheep depended on the category of histological lesions. AGID and ELISA were conditionally independent, and appeared to detect overlapping but distinct subgroups of infected animals. The ELISA was significantly more sensitive than the AGID. The ELISA was simple to perform, robust and repeatable. Coefficients of variation of <12.0% were observed for positive and negative controls included on 193 plates over a 10-month period and there was a high level of intraassay repeatability with 12.0% of the duplicate samples having CV of >15.0%.
本研究旨在评估一种商业化酶联免疫吸附测定试剂盒（Institut Pourquier）在澳大利亚条件下诊断绵羊和山羊副结核病的性能，并将其准确性与现有的琼脂凝胶免疫扩散试验（AGID）进行比较。该酶联免疫吸附测定法在绵羊和山羊中的敏感性分别为34.9%和56.4%，特异性分别为98.8%和100.0%。AGID在绵羊中的敏感性为13.8%，在山羊中为39.5%，两种动物的特异性均为100.0%。酶联免疫吸附测定法在绵羊中的敏感性取决于组织学病变类别。AGID和酶联免疫吸附测定法条件独立，且似乎能检测出感染动物中重叠但不同的亚组。酶联免疫吸附测定法比AGID明显更敏感。该酶联免疫吸附测定法操作简单、稳健且可重复。在10个月期间对193个平板上的阳性和阴性对照进行观察，变异系数<12.0%，并且12.0%的重复样品的批内变异系数>15.0%，具有较高的批内重复性。
