来自变铅青链霉菌66的CelB内切葡聚糖酶的纯化与特性鉴定及编码基因的DNA序列
Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene.
作者信息
Wittmann S, Shareck F, Kluepfel D, Morosoli R
机构信息
Centre de Recherche en Microbiologie Appliquée, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Canada.
出版信息
Appl Environ Microbiol. 1994 May;60(5):1701-3. doi: 10.1128/aem.60.5.1701-1703.1994.
Abstract
The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the Vmax and Km values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H family of cellulases.
摘要
从淡紫链霉菌IAF9培养滤液中分离出的内切葡聚糖酶CelB的相对分子质量为36000。以羧甲基纤维素为底物时，Vmax和Km值分别为110 IU/mg酶和1.3 mg/ml。通过对氨基酸一级序列的比较，CelB被归类于纤维素酶的H家族。
Zotero 插件