Armstrong L C, Watkins K, Pinkerton K E, Last J A
Department of Internal Medicine (Pulmonary), University of California, Davis 95616-8542.
Am J Respir Cell Mol Biol. 1994 Jul;11(1):25-34. doi: 10.1165/ajrcmb.11.1.8018336.
cDNAs, synthesized by the polymerase chain reaction (PCR) technique, were used to quantify mRNA concentrations for the alpha 1 (I) and alpha 1 (III) chains of collagen types I and III and for beta-actin in the lungs of rats exposed to either filtered air or to 1.2 ppm of ozone. The alpha 1 (I) procollagen mRNA concentration was increased by about 30% in the lungs of rats exposed to ozone, while the concentrations of the mRNAs for alpha 1 (III) procollagen and for beta-actin were the same in the lungs of control and ozone-exposed animals. The lungs from the rats exposed to 1.2 ppm of ozone preferentially synthesized type I collagen as compared with controls. Sites of increased expression of the alpha 1 (I) procollagen mRNA were detected by in situ hybridization in lung sections embedded in paraffin prepared from rats exposed either to filtered air or to ozone. The lungs from rats treated with ozone focally expressed increased amounts of alpha 1 (I) procollagen mRNA in the lung parenchyma at the septal tips and the bronchiole-alveolar duct junctions. They also showed an apparent diffuse increase in alpha 1 (III) procollagen mRNA expression. We conclude that exposure of rats to high concentrations of ozone causes a specific increase in the lung content of mRNA for the major chain of type I collagen.
通过聚合酶链反应（PCR）技术合成的互补DNA（cDNA），用于定量暴露于过滤空气或1.2 ppm臭氧的大鼠肺中I型和III型胶原蛋白的α1（I）和α1（III）链以及β-肌动蛋白的mRNA浓度。暴露于臭氧的大鼠肺中α1（I）前胶原mRNA浓度增加了约30%，而对照动物和暴露于臭氧的动物肺中α1（III）前胶原和β-肌动蛋白的mRNA浓度相同。与对照相比，暴露于1.2 ppm臭氧的大鼠肺优先合成I型胶原蛋白。通过原位杂交在从暴露于过滤空气或臭氧的大鼠制备的石蜡包埋的肺切片中检测α1（I）前胶原mRNA表达增加的部位。用臭氧处理的大鼠肺在肺实质的间隔尖端和细支气管-肺泡管交界处局部表达增加量的α1（I）前胶原mRNA。它们还显示α1（III）前胶原mRNA表达明显弥漫性增加。我们得出结论，大鼠暴露于高浓度臭氧会导致I型胶原蛋白主要链的肺mRNA含量特异性增加。
