Klaase J M, Kroon B B, Beijnen J H, van Slooten G W, van Dongen J A
Department of Surgery, The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis), Amsterdam.
Br J Cancer. 1994 Jul;70(1):151-3. doi: 10.1038/bjc.1994.266.
In 14 consecutive patients with recurrent melanoma of the lower limb a total of 35 biopsies were taken at the end of perfusion treatment to assess melphalan tissue concentrations in tumour, skin/subcutis and muscle tissue. In tumour tissue (n = 12) the mean melphalan concentration was 6.8 micrograms g-1, which was significantly higher than that of healthy skin/subcutis (3.2 micrograms g-1; n = 10), but equal to that of muscle tissue (6.5 micrograms g-1; n = 13). The correlation between melphalan concentration in the tissues and the concentration in the perfusate was studied. The latter was assessed in the form of melphalan peak concentration and the area under the curve (AUC0-->60) of the melphalan concentration-time curve. Tumour concentration proved to be correlated linearly with AUC0-->60 (R = 0.6, P = 0.002) and muscle concentration with melphalan peak concentration (R = 0.8, P = 0.04). There was no relation between skin/subcutis concentrations and the perfusate parameters. Further research is warranted to study the relationship between melphalan tissue concentration, tumour response and regional toxicity.
在14例复发性下肢黑色素瘤患者中，在灌注治疗结束时共进行了35次活检，以评估肿瘤、皮肤/皮下组织和肌肉组织中马法兰的组织浓度。在肿瘤组织（n = 12）中，马法兰的平均浓度为6.8微克/克，显著高于健康皮肤/皮下组织（3.2微克/克；n = 10），但与肌肉组织（6.5微克/克；n = 13）的浓度相当。研究了组织中马法兰浓度与灌注液中浓度之间的相关性。后者以马法兰峰值浓度和马法兰浓度-时间曲线的曲线下面积（AUC0→60）的形式进行评估。结果证明肿瘤浓度与AUC0→60呈线性相关（R = 0.6，P = 0.002），肌肉浓度与马法兰峰值浓度相关（R = 0.8，P = 0.04）。皮肤/皮下组织浓度与灌注液参数之间没有关系。有必要进一步研究马法兰组织浓度、肿瘤反应和局部毒性之间的关系。
