Dutra W O, Martins-Filho O A, Cançado J R, Pinto-Dias J C, Brener Z, Freeman Júnior G L, Colley D G, Gazzinelli G, Parra J C
Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Brazil.
Int Immunol. 1994 Apr;6(4):499-506. doi: 10.1093/intimm/6.4.499.
Whole blood preparations from patients with either the indeterminate (asymptomatic) or cardiac clinical forms of chronic Trypanosoma cruzi infection were analyzed by flow cytometry using double-labeling to identify subsets of circulating lymphocytes. Several significant differences were demonstrated between the blood lymphocyte profiles of chagasic patients and non-chagasic controls. Clear increase in the percentages and actual numbers of double-positive cells of the phenotype CD3+/HLA-DR+, as well as decrease in the percentage of CD45RA+/CD4+ and CD45RA+/CD8+ T cells, indicate greater numbers of activated T cells circulating in the blood of infected patients. Consistent parallel increases were seen also in the B lymphocyte subset which stained double-positive for CD19/CD5. There were no significant differences in the circulation of these chronic chagasic patients in the CD4:CD8 ratios. Also, no substantive phenotypic differences were observed in the lymphocyte populations between the two ends of the clinical spectrum (indeterminate versus cardiac) in chronic human Chagas' disease. These observations demonstrate that increased levels of activated T cells and CD5+ B cells are present in the circulation of people with chronic Chagas' disease. These are cell phenotypes that have been associated in other conditions with autoimmune, polyclonal, and hyperimmune responses. The specificities of these activated cells and the roles they may play in resistance or pathogenesis during chronic Chagas' disease need now to be determined.
采用双标记流式细胞术分析慢性克氏锥虫感染的不确定型（无症状型）或心脏临床型患者的全血制剂，以识别循环淋巴细胞亚群。查加斯病患者与非查加斯病对照者的血液淋巴细胞谱存在若干显著差异。CD3+/HLA-DR+表型双阳性细胞的百分比和实际数量明显增加，以及CD45RA+/CD4+和CD45RA+/CD8+T细胞百分比降低，表明感染患者血液中循环的活化T细胞数量更多。CD19/CD5双阳性染色的B淋巴细胞亚群也呈现一致的平行增加。这些慢性查加斯病患者的CD4:CD8比值在循环中无显著差异。此外，在慢性人类恰加斯病临床谱两端（不确定型与心脏型）的淋巴细胞群体中未观察到实质性的表型差异。这些观察结果表明，慢性恰加斯病患者循环中存在活化T细胞和CD5+B细胞水平升高的情况。这些是在其他情况下与自身免疫、多克隆和超免疫反应相关的细胞表型。现在需要确定这些活化细胞的特异性及其在慢性恰加斯病抵抗或发病机制中可能发挥的作用。
