Interactions of polyamines in the measurement of free magnesium concentration by mag-fura-2 and 31P-NMR.
作者信息
Günther T, Vormann J, Konstanczak P, Schäfer A
机构信息
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Germany.
出版信息
Biochim Biophys Acta. 1994 Jun 22;1192(2):281-5. doi: 10.1016/0005-2736(94)90129-5.
PMID:8018709
Abstract
Polyamines, particularly spermine, in physiological concentrations interact with mag-fura-2 and the mag-fura-2/Mg2+ complex, resulting in reduced values of free Mg2+ concentration. Similarly, polyamines interact with ATP and MgATP. Thus, free Mg2+ concentration, as measured by 31P-NMR or mag-fura-2, is underestimated in the presence of polyamines, particularly of spermine.
摘要
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