MgATP化学位移极限校准中的误差：对通过31P NMR光谱法测定游离镁的影响。
Error in the calibration of the MgATP chemical-shift limit: effects on the determination of free magnesium by 31P NMR spectroscopy.
作者信息
Mosher T J, Williams G D, Doumen C, LaNoue K F, Smith M B
机构信息
Department of Radiology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033.
出版信息
Magn Reson Med. 1992 Mar;24(1):163-9. doi: 10.1002/mrm.1910240117.
The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical-shift limits delta ATP alpha beta and delta MgATP alpha beta. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of delta MgATP alpha beta. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate delta MgATP alpha beta.
利用ATP的31P化学位移测量细胞内游离镁离子（Mg2+）需要使用合适的校准溶液来确定化学位移极限δATPαβ和δMgATPαβ。含有过量Mg2+的溶液含有大量的Mg2ATP，会导致δMgATPαβ值出现正误差。对于生理应用而言，这可能会使细胞内游离Mg2+的估计值高估多达300%。如果使用合适的Mg2+/ATP摩尔比来校准δMgATPαβ，这种误差可能会降至最低。
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