葡萄球菌核酸酶中四键HN-Hα J耦合的测量。
Measurement of four-bond HN-H alpha J-couplings in staphylococcal nuclease.
作者信息
Vuister G W, Bax A
机构信息
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
出版信息
J Biomol NMR. 1994 Mar;4(2):193-200. doi: 10.1007/BF00175247.
Quantitative J-correlation and triple-resonance ECOSY-type experiments are used to unambiguously establish the presence of four-bond sequential HN-H alpha J-couplings in the protein staphylococcal nuclease. Substantially negative 4JH alpha HN values, ranging from -0.8 to -2.3 Hz, are observed when the psi angle is near +120 degrees, and the following phi angle near +60 degrees. For other conformations, the four-bond HN-H alpha J-couplings fall between -0.5 and +0.5 Hz.
定量J-相关和三共振ECOSY型实验被用于明确证实蛋白质葡萄球菌核酸酶中四键顺序的HN-Hα J-耦合的存在。当ψ角接近+120度且随后的φ角接近+60度时，观察到显著为负的4JHαHN值，范围为-0.8至-2.3赫兹。对于其他构象，四键HN-Hα J-耦合在-0.5至+0.5赫兹之间。
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