Villers D, Milpied N, Gaschet J, Davodeau F, Hallet M M, Bonneville M, Vié H
INSERM U211 and CHR Nantes, Institut de Biologie, France.
Bone Marrow Transplant. 1994 Jan;13(1):19-26.
We investigated T cell receptor (TCR) alpha/beta and gamma/delta repertoire reconstitution after autologous and allogeneic bone marrow transplantation (BMT) in humans using 13 monoclonal antibodies directed at constant and variable regions of the TCR. The TCR gamma/delta repertoire was studied kinetically during the first month and then 1 year post-BMT whereas alpha/beta peripheral blood T lymphocytes (PBTL) were studied within the first 3 months and 1 year post-BMT. Through these two studies, we found 7 of 26 patients with over-represented TCR gamma/delta subsets during the early period post-BMT. Moreover, during this period the V gamma 9V delta 2 frequency among gamma/delta T cells was found to be higher than among normal donors. Study on TCR alpha/beta T cells also revealed abnormally expanded V-specific subset (5 of 10 patients within 3 months following BMT) demonstrating that repertoire alteration early after BMT is a general phenomenon concerning potentially all T cell subsets. More surprisingly, the alpha/beta T cell repertoire was also found to be altered late after BMT (7 of 15 patients after 1 year post-BMT presented one or more overepresented alpha/beta TCR subset). These alterations of TCR combinatorial diversity should be taken into account in understanding the immunological status of patients after BMT.
我们使用13种针对T细胞受体（TCR）恒定区和可变区的单克隆抗体，研究了人类自体和异体骨髓移植（BMT）后T细胞受体α/β和γ/δ库的重建情况。在BMT后的第一个月以及之后的1年中动态研究了TCRγ/δ库，而α/β外周血T淋巴细胞（PBTL）则在BMT后的前3个月和1年进行研究。通过这两项研究，我们发现26例患者中有7例在BMT后的早期出现TCRγ/δ亚群过度表达。此外，在此期间，γ/δT细胞中Vγ9Vδ2频率高于正常供体。对TCRα/βT细胞的研究还发现V特异性亚群异常扩增（BMT后3个月内10例患者中有5例），表明BMT后早期库的改变是涉及所有潜在T细胞亚群的普遍现象。更令人惊讶的是，还发现BMT后晚期α/βT细胞库也发生了改变（BMT后1年的15例患者中有7例出现一个或多个α/βTCR亚群过度表达）。在理解BMT后患者的免疫状态时，应考虑TCR组合多样性的这些改变。
