Hoshino T, Ohta A, Nakao M, Ota T, Inokuchi T, Matsueda S, Gouhara R, Yamada A, Itoh K, Oizumi K
First Department of Internal Medicine, Kurume University, Japan.
J Rheumatol. 1996 Jan;23(1):124-9.
Adult Still's disease, a systemic inflammatory disorder of unknown etiology, shows few specific laboratory markers and histological features. We investigated T cell subsets in peripheral blood lymphocytes (PBL) of patients with adult Still's disease to elucidate immunological features of the disease.
We studied T cell subsets in PBL of 12 patients with adult Still's disease by flow cytometric analysis. T cell subsets were also investigated for more than 6 months in PBL of 3 patients with adult Still's disease.
Both the percentage and absolute number of T cell receptor gamma delta positive (TCR gamma delta +) T cells in active adult Still's disease (n = 6) were significantly (p < 0.05) higher than those in inactive adult Still's disease (n = 6), inactive rheumatoid arthritis (n = 8), or healthy controls (n = 20). An increase of TCR gamma delta + T cells was observed in 5 of 6 patients with adult Still's disease at the active phase. TCR gamma delta + T cells also increased for a considerable period in all the patients tested. In contrast, there were no significant differences in the other T cell subsets (CD3, CD4, CD8, TCR alpha beta) between all the patients and healthy donors. Levels of TCR gamma delta + T cells in PBL correlated well with those of serum ferritin and C-reactive protein in one of the 3 patients whose PBL could be serially investigated. These increased TCR gamma delta + T cells mostly consisted of a V gamma 9/V delta 2 subset.
TCR gamma delta + T cells in PBL are significantly increased in the active phase in patients with adult Still's disease.
成人斯蒂尔病是一种病因不明的全身性炎症性疾病，几乎没有特异性的实验室指标和组织学特征。我们研究了成人斯蒂尔病患者外周血淋巴细胞（PBL）中的T细胞亚群，以阐明该疾病的免疫特征。
我们通过流式细胞术分析研究了12例成人斯蒂尔病患者PBL中的T细胞亚群。还对3例成人斯蒂尔病患者的PBL进行了6个多月的T细胞亚群研究。
活动期成人斯蒂尔病患者（n = 6）中T细胞受体γδ阳性（TCRγδ +）T细胞的百分比和绝对数量均显著高于非活动期成人斯蒂尔病患者（n = 6）、非活动期类风湿关节炎患者（n = 8）或健康对照者（n = 20）（p < 0.05）。6例活动期成人斯蒂尔病患者中有5例观察到TCRγδ + T细胞增加。在所有测试患者中，TCRγδ + T细胞也在相当长的一段时间内增加。相比之下，所有患者与健康供体之间的其他T细胞亚群（CD3、CD4、CD8、TCRαβ）没有显著差异。在3例PBL可连续检测的患者中，有1例患者PBL中TCRγδ + T细胞水平与血清铁蛋白和C反应蛋白水平密切相关。这些增加的TCRγδ + T细胞主要由Vγ9/Vδ2亚群组成。
成人斯蒂尔病患者活动期PBL中的TCRγδ + T细胞显著增加。
