Harkin D G, Bignold L P
Department of Pathology, University of Adelaide, Australia.
Cell Biol Int. 1994 Mar;18(3):177-87. doi: 10.1006/cbir.1994.1059.
Human neutrophils suspended in Hanks' balanced salt solution (37 degrees C, 20 mM Hepes, pH 7.2) produced extensions, elongated and developed a polarised morphology with both a pseudopod and uropod when exposed to C5a (10 nM), leukotriene B4 (10 nM), platelet activating factor (40 nM) or interleukin-8 (12.5 nM). Responses to each mediator were generally enhanced or unaffected by chelators of extracellular Ca2+ and Mg2+. Neutrophils suspended in heparinised plasma (90-10% v/v in Hanks' balanced salt solution) produced extensions, elongated and developed a pseudopod, but rarely developed a uropod unless additional Mg2+ ions (0.5-5 mM) were added. These findings demonstrate that the polarisation of neutrophils in plasma is significantly different to that induced by endogenous chemoattractants with regard to the frequency of uropod formation and requirement for extracellular divalent cations.
悬浮于汉克斯平衡盐溶液（37℃，20mM Hepes，pH 7.2）中的人中性粒细胞，在暴露于C5a（10nM）、白三烯B4（10nM）、血小板活化因子（40nM）或白细胞介素-8（12.5nM）时，会产生突起，细胞伸长并形成具有伪足和尾足的极化形态。细胞外Ca2+和Mg2+的螯合剂通常会增强或不影响对每种介质的反应。悬浮于肝素化血浆（在汉克斯平衡盐溶液中体积比为90 - 10%）中的中性粒细胞会产生突起，细胞伸长并形成伪足，但除非添加额外的Mg2+离子（0.5 - 5mM），否则很少形成尾足。这些发现表明，就尾足形成的频率和对细胞外二价阳离子的需求而言，血浆中中性粒细胞的极化与内源性趋化因子诱导的极化有显著差异。
