Molinari M, Leggio M G, Dell'Anna M E, Giannetti S, Macchi G
Experimental Neurology Laboratory, Catholic University, Rome, Italy.
Eur J Neurosci. 1994 Mar 1;6(3):299-312. doi: 10.1111/j.1460-9568.1994.tb00273.x.
Neurons projecting to the parietal cortex or striatum and neurons showing immunoreactivity for the calcium-binding proteins parvalbumin and 28KD-calbindin were examined in the anterior intralaminar nuclei (IL) of the cat. Retrograde tracing from deep or superficial parietal cortical layers or from the caudate nucleus was coupled with immunohistochemistry to determine which of these proteins were expressed in the projection neurons. It was found that IL neurons project to deep as well as to superficial layers of the parietal cortex, that IL-cortical neurons could be differentiated into two populations according to their cortical projection pattern and their soma size, and that IL neurons projecting to the parietal cortex or to the striatum express 28KD calbindin immunoreactivity but not parvalbumin immunoreactivity. The distribution of immunoreactivity to 28KD calbindin and parvalbumin in the neuropil showed a consistent complementary distribution pattern in the IL. The compartments based on differential parvalbumin and 28KD calbindin expression may indicate the presence of functionally segregated units in IL.
在猫的前内侧核（IL）中，研究了投射到顶叶皮层或纹状体的神经元以及对钙结合蛋白小白蛋白和28KD钙结合蛋白呈免疫反应性的神经元。从顶叶皮层深层或浅层或尾状核进行逆行追踪，并结合免疫组织化学，以确定这些蛋白质中哪些在投射神经元中表达。研究发现，IL神经元投射到顶叶皮层的深层和浅层，IL-皮层神经元可根据其皮层投射模式和胞体大小分为两类，投射到顶叶皮层或纹状体的IL神经元表达28KD钙结合蛋白免疫反应性，但不表达小白蛋白免疫反应性。在神经毡中，28KD钙结合蛋白和小白蛋白的免疫反应性分布在IL中呈现出一致的互补分布模式。基于小白蛋白和28KD钙结合蛋白表达差异的区室可能表明IL中存在功能上分离的单元。
