Novak A, Kruskic M, Ludoski M, Jurukovski V
Cytogenetics Laboratory, Institute of Hematology University Clinical Center, Belgrade, Yugoslavia.
Cancer Genet Cytogenet. 1994 Jun;74(2):109-14. doi: 10.1016/0165-4608(94)90007-8.
The original impetus for this work was the need for a method to analyze chromosome abnormalities in patients with hematopoietic neoplasms immediately after bone marrow (BM) aspiration. We present an easy and reproducible procedure for obtaining G-bands simultaneously with chromosome preparations (utilizing trypsin through hypotonic shock). It provides chromosomes of good quality with satisfactory banding range (450-500) within only 6 hours after BM aspiration. Using this technique, in our series of 560 patients with preleukemia and leukemia, we consistently provided the banding quality that allowed all chromosomes of the karyotype to be identified in 96% of myelodysplastic syndromes (MDS) 91% of acute lymphocytic leukemia (ALL), and 94% of acute myeloid leukemia (AML) cases, and we also identified cytogenetically abnormal clones in 70% of AML patients. With the same success this technique is also applicable to other types of human cells: lymphocytes from peripheral blood (PB) and established cell lines. Furthermore, this technique conserves chromatin structure and permits high hybridization efficiency rate on prebanded chromosomes and identification of chromosome markers within 36 hours after BM aspirations.
这项工作的最初动力是需要一种方法，以便在骨髓（BM）抽吸后立即分析造血系统肿瘤患者的染色体异常情况。我们提出了一种简单且可重复的程序，在制备染色体的同时获得G带（通过低渗休克使用胰蛋白酶）。该程序在BM抽吸后仅6小时内就能提供质量良好、带纹范围令人满意（450 - 500）的染色体。使用这项技术，在我们的560例白血病前期和白血病患者系列中，我们始终能提供使核型中的所有染色体在96%的骨髓增生异常综合征（MDS）、91%的急性淋巴细胞白血病（ALL）和94%的急性髓系白血病（AML）病例中得以识别的带纹质量，并且我们还在70%的AML患者中识别出细胞遗传学异常克隆。这项技术同样成功地适用于其他类型的人类细胞：外周血（PB）淋巴细胞和已建立的细胞系。此外，这项技术保留了染色质结构，并在BM抽吸后36小时内允许对预带纹染色体进行高效杂交以及识别染色体标记。
