Michalová K, Cermák J, Brezinová J, Zemanová Z
Institute of Hematology and Blood Transfusion, General Faculty Hospital, Prague, Czech Republic.
Cancer Genet Cytogenet. 1999 Feb;109(1):76-8. doi: 10.1016/s0165-4608(98)00133-2.
Presence of double minute chromosomes (dmin) is rare in bone marrow cells in patients with preleukemia and leukemia. We describe a case of myelodysplastic syndrome-refractory anemia with excess of blasts (MDS-RAEB) associated with two unrelated pathological chromosomal clones that developed during the progression of the disease. The patient was followed cytogenetically for a period of 4 years. At the time of transition into RAEB-T and later to acute myeloid leukemia (AML), dmin were associated with resistance to chemotherapy. Fluorescence in situ hybridization study proved that the dmin in this case were c-MYC amplicons. At the terminal stage of the disease, dmins were present in all 50 analyzed cells.
双微体染色体（dmin）在白血病前期和白血病患者的骨髓细胞中很少见。我们描述了一例伴有过多原始细胞的骨髓增生异常综合征难治性贫血（MDS-RAEB）病例，该病例与疾病进展过程中出现的两个不相关的病理性染色体克隆相关。对该患者进行了4年的细胞遗传学随访。在转变为RAEB-T以及后来转变为急性髓系白血病（AML）时，dmin与化疗耐药相关。荧光原位杂交研究证明，该病例中的dmin是c-MYC扩增子。在疾病末期，所有50个分析细胞中均存在dmin。
