Gruszczynska M, Thang T C, Csaba G
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
Cytobios. 1994;77(310):167-74.
Liver cells of rats given a single insulin dose at birth (imprinted) do not alter from the controls at 1 week postnatal. When hormone treatment occurs at 1 week postnatal without any prior administration of it, an increase in glycogen and lipid with nuclear deposition of the latter can be seen. The cell surface displays a dense layer, and increased micropinocytosis is accompanied by an increase in the number of coated pits and vesicles. Liver cells of imprinted animals treated repeatedly at 1 week postnatal have much more glycogen and lipid, and more prominent endoplasmic reticulum but a less mature Golgi apparatus. The dense substance of the cell surface is missing and the signs of endocytosis are rarely seen. These alterations indicate the influence of imprinting on hormone binding and possibly on the next steps after interaction in the cell.
出生时接受单次胰岛素剂量（印记）的大鼠肝细胞在出生后1周时与对照组相比没有变化。当在出生后1周进行激素治疗且之前未进行任何激素给药时，可以看到糖原增加以及脂质增加且脂质出现核沉积。细胞表面呈现致密层，微胞饮作用增加伴随着有被小窝和小泡数量的增加。在出生后1周反复接受治疗的印记动物的肝细胞含有更多的糖原和脂质，内质网更显著，但高尔基体不太成熟。细胞表面的致密物质缺失，内吞作用的迹象很少见。这些改变表明印记对激素结合以及可能对细胞内相互作用后的后续步骤有影响。
