Liang H, Wright W H, Rieder C L, Salmon E D, Profeta G, Andrews J, Liu Y, Sonek G J, Berns M W
Beckman Laser Institute and Medical Clinic, University of California, Irvine 92715.
Exp Cell Res. 1994 Jul;213(1):308-12. doi: 10.1006/excr.1994.1203.
A pulsed-laser microbeam at 532 nm wavelength (optical scissors) and a laser-induced optical trap at 1064 nm wavelength (optical tweezers) have been successively combined to dissect and manipulate chromosomes in live newt lung epithelial cells. These preliminary experimental results demonstrated that chromosome fragments dissected by laser microbeam surgery, regardless of their size, could be easily pulled or rotated by optical forces when positioned at the periphery of the mitotic spindle. In addition, chromosome arms which were not subjected to laser microsurgery also could be moved with the optical tweezers at the spindle periphery. In our previous study on rat kangaroo kidney cells (PTK2), this degree of facilty in manipulating chromosome movement was not possible, most likely due to the close proximity of the intermediate filament "cage" to the spindle. It is concluded herein that optical scissors and tweezers can be used in combination to study the interaction of chromosomes with the mitotic spindle in cells where the peripheral regions of the spindle are unobstructed by intermediate filaments. This can be performed on newt cells, where the diameter of the cage can be substantially larger than the diameter of the spindle.
波长为532纳米的脉冲激光微束（光学剪刀）和波长为1064纳米的激光诱导光学陷阱（光镊）已被相继组合，用于解剖和操纵蝾螈肺上皮活细胞中的染色体。这些初步实验结果表明，通过激光微束手术解剖出的染色体片段，无论其大小如何，当位于有丝分裂纺锤体周边时，都能很容易地被光力拉动或旋转。此外，未接受激光显微手术的染色体臂也能用光镊在纺锤体周边移动。在我们之前对大鼠袋鼠肾细胞（PTK2）的研究中，无法达到这种操纵染色体移动的便利程度，这很可能是由于中间丝“笼”与纺锤体靠得很近所致。本文得出结论，光学剪刀和光镊可联合用于研究纺锤体周边区域未被中间丝阻碍的细胞中染色体与有丝分裂纺锤体的相互作用。这可以在蝾螈细胞上进行，因为蝾螈细胞中“笼”的直径可比纺锤体的直径大得多。
