Hoppert M, Braks I, Mayer F
Institut für Mikrobiologie, Georg-August-Universität Göttingen, FRG.
FEMS Microbiol Lett. 1994 May 15;118(3):249-54. doi: 10.1111/j.1574-6968.1994.tb06836.x.
In water-in-oil microemulsion the membrane-associated F420-hydrogenase of Methanobacterium thermoautotrophicum (strain Marburg) and the membrane-bound hydrogenase of Alcaligenes eutrophus H 16 (MBH) showed prolonged activity at elevated temperatures (measured as hydrogen production) as compared to aqueous buffer solution. The temperature optimum of the reactions was about 15 degrees C higher than in aqueous buffer solution. Activity of the almost completely inactivated F420-hydrogenase could be partially recovered by transfer into microemulsion.
