Aerssens J, Chaffanet M, Baens M, Matthijs G, Van den Berghe H, Cassiman J J, Marynen P
Arthritis and Metabolic Bone Disease Research Unit, Center for Human Genetics-K.U., Leuven, Belgium.
Genomics. 1994 Mar 1;20(1):119-21. doi: 10.1006/geno.1994.1136.
Two somatic cell hybrids containing the der(12) or the der(X) from a mesothelioma with a translocation t(X;12)(q22;p13) as the only chromosomal change were generated to characterize the region of 12p13 containing the translocation breakpoint. Fluorescence in situ hybridization analysis showed the breakpoint on chromosome 12 to occur between VWF and D12S158. On the linkage map developed by J. Weissenbach et al. (Nature, 1992, 359: 794-801), the breakpoints were located between D12S99 and D12S100 on chromosome 12 and between DXS1106 and DXS1001 on chromosome X. PCR analysis based on genomic sequences, with DNA from both somatic cell hybrids, enabled us to map CACNL1A1, FGF6, D12S370, D12S38OE, D12S381E, and D12S382E distally to the 12p13 breakpoint and to VWF.
通过一个间皮瘤的t(X;12)(q22;p13)易位作为唯一染色体改变，产生了两个分别含有der(12)或der(X)的体细胞杂种，以对包含易位断点的12p13区域进行特征分析。荧光原位杂交分析显示12号染色体上的断点位于VWF和D12S158之间。在J. 魏森巴赫等人（《自然》，1992年，359卷：794 - 801页）绘制的连锁图谱上，断点位于12号染色体的D12S99和D12S100之间以及X染色体的DXS1106和DXS1001之间。基于基因组序列的PCR分析，利用两个体细胞杂种的DNA，使我们能够将CACNL1A1、FGF6、D12S370、D12S38OE、D12S381E和D12S382E定位到12p13断点和VWF的远端。
