Michalski A J, Cowell J K
ICRF Oncology Unit, Institute of Child Health, London, United Kingdom.
Genomics. 1993 Oct;18(1):141-3. doi: 10.1006/geno.1993.1439.
To define the position of a 13q12 breakpoint from a patient with ganglioneuroblastoma, a series of somatic cell hybrids carrying human chromosome translocations with breakpoints in the proximal part of chromosome 13 has been compiled. Sequence-tagged sites (STS) have been generated from a series of Alu-PCR probes previously shown to be in the 13q12 region. Together with an STS for the oncogene FLT1, these have been used to define the relative positions of the translocation breakpoints in the hybrids. In this way, four markers have been ordered in three subregions of 13q12 and reference breakpoints established. The refined physical map of 13q12 provides a series of reference markers with known locations and will be invaluable in the further characterization of breakpoints in this region.
为了确定一名神经节神经母细胞瘤患者13q12断点的位置，我们构建了一系列携带人类染色体易位且断点位于13号染色体近端的体细胞杂种。从先前显示位于13q12区域的一系列Alu-PCR探针中生成了序列标签位点（STS）。连同癌基因FLT1的一个STS一起，这些已被用于确定杂种中易位断点的相对位置。通过这种方式，在13q12的三个子区域中对四个标记进行了排序并建立了参考断点。13q12的精细物理图谱提供了一系列位置已知的参考标记，这对于该区域断点的进一步表征将是非常宝贵的。
