Scott A F, Elizaga A, Morrell J, Bergen A, Penno M B
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-4925.
Genomics. 1994 Mar 15;20(2):227-30. doi: 10.1006/geno.1994.1157.
We report the sequence and genomic organization of a gene linked to Ki-ras in the mouse and coamplified in Y1 murine adrenal carcinoma cells. The entire 4.4-kb cDNA sequence as well as promoter and splice sites for each of the three exons was determined. The gene, designated KRAG (Ki-ras-associated gene) has a CG-rich first exon and promoter region and a long 3' untranslated region and encodes 216 amino acids. The putative 23.9-kDa protein has four potential transmembrane hydrophobic domains. The hydropathy plot resembles that of certain tumor-associated antigens, including CO-029 and ME491. A potential human homologue, EST05985, was identified and provisionally mapped to human chromosome 12, a chromosome syntenic to mouse chromosome 6, the previously mapped location of KRAG.
我们报道了与小鼠Ki-ras基因相连且在Y1鼠肾上腺癌细胞中共同扩增的一个基因的序列和基因组结构。测定了整个4.4kb的cDNA序列以及三个外显子各自的启动子和剪接位点。该基因命名为KRAG（Ki-ras相关基因），其第一个外显子和启动子区域富含CG，3'非翻译区长，编码216个氨基酸。推测的23.9kDa蛋白有四个潜在的跨膜疏水结构域。亲水性图谱类似于某些肿瘤相关抗原，包括CO-029和ME491。鉴定出一个潜在的人类同源物EST05985，并初步定位到人类12号染色体，该染色体与小鼠6号染色体同线，而KRAG先前定位在小鼠6号染色体上。
