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异源双链模板在RNA聚合酶II激活转录过程中规避了对ATP的能量需求。

A heteroduplex template circumvents the energetic requirement for ATP during activated transcription by RNA polymerase II.

作者信息

Tantin D, Carey M

机构信息

Molecular Biology Institute, UCLA School of Medicine 90024.

出版信息

J Biol Chem. 1994 Jul 1;269(26):17397-400.

PMID:8021241
Abstract

Unlike most eukaryotic and prokaryotic RNA polymerases, promoter-specific transcription by RNA polymerase II requires hydrolysis of the ATP beta-gamma phosphoanhydride bond. Here we show that a template containing a 10-base pair DNA mismatch encompassing the start site circumvents this requirement such that the non-hydrolyzable ATP analogues, ATP gamma S (adenosine 5'-O-(thiotriphosphate)) and AMP-PNP (adenyl-5'-yl imidodiphosphate), support both basal and GAL4-VP16-activated transcription in a reconstituted HeLa cell in vitro transcription system. The results imply that ATP regulates either opening of the template at the start site or a closely associated step.

摘要

与大多数真核生物和原核生物的RNA聚合酶不同，RNA聚合酶II的启动子特异性转录需要ATPβ-γ磷酸酐键的水解。我们在此表明，包含围绕起始位点的10个碱基对DNA错配的模板规避了这一要求，使得不可水解的ATP类似物ATPγS（腺苷5'-O-（硫代三磷酸））和AMP-PNP（腺苷-5'-基亚氨基二磷酸）在重构的HeLa细胞体外转录系统中支持基础转录和GAL4-VP16激活的转录。这些结果表明，ATP调节起始位点处模板的打开或紧密相关的步骤。

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J Biol Chem. 1994 Jul 1;269(26):17397-400.
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