Lymn J S, Godfrey N P, Thurston H
Department of Medicine, University of Leicester, UK.
J Hypertens. 1994 Feb;12(2):129-35.
To examine the changes in the rate of DNA synthesis in the aorta and mesenteric and subcutaneous resistance arteries during the development of renovascular hypertension.
Goldblatt two-kidney, one clip hypertensive and sham-operated control rats were studied 3, 7, 14 and 28 days after surgery. DNA synthesis was measured as the hourly rate of [3H]-thymidine incorporation into the DNA.
Three days after renal artery constriction there was a significant increase in the DNA synthesis in both the aorta and the mesenteric vessels, although the blood pressure was not changed. DNA synthesis was elevated during the development of hypertension, but returned to control levels after 28 days when the blood pressure had reached a plateau. By contrast, there was no increase of DNA synthesis in the subcutaneous vessels at any time after renal artery constriction. The plasma renin concentration also was increased after 3 days in the clipped rats and remained elevated throughout the study. There were no significant changes in the blood pressure, the plasma renin concentration or the rate of vascular DNA synthesis in the sham-operated control rats.
These data indicate that there are regional differences in the vascular response to the induction of renovascular hypertension and that in some vascular beds an increase in DNA synthesis precedes the rise in blood pressure.
研究肾血管性高血压发展过程中主动脉、肠系膜动脉和皮下阻力动脉的DNA合成速率变化。
对Goldblatt二肾一夹型高血压大鼠及假手术对照大鼠在术后3、7、14和28天进行研究。DNA合成通过[³H] - 胸腺嘧啶核苷掺入DNA的每小时速率来测定。
肾动脉缩窄3天后，尽管血压未改变，但主动脉和肠系膜血管的DNA合成显著增加。在高血压发展过程中DNA合成升高，但在血压达到稳定水平的28天后恢复至对照水平。相比之下，肾动脉缩窄后皮下血管的DNA合成在任何时候均未增加。夹闭大鼠术后3天血浆肾素浓度也升高，并在整个研究过程中持续升高。假手术对照大鼠的血压、血浆肾素浓度或血管DNA合成速率无显著变化。
这些数据表明，肾血管性高血压诱导过程中血管反应存在区域差异，并且在一些血管床中，DNA合成增加先于血压升高。
