Willcocks M M, Brown T D, Madeley C R, Carter M J
Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, U.K.
J Gen Virol. 1994 Jul;75 ( Pt 7):1785-8. doi: 10.1099/0022-1317-75-7-1785.
We have determined the complete genomic sequence of human astrovirus serotype 1 isolated in Newcastle upon Tyne. The genome is 6813 nucleotides long and contains three sequential open reading frames (ORFs). The two closest to the 5' end are linked by a ribosomal frameshifting motif and contain sequence motifs indicative of non-structural virus proteins: serine protease and RNA-dependent RNA polymerase. A nuclear addressing sequence is also located here. The 3' ORF encodes the virion structural polypeptides as a polyprotein precursor. This genomic organization resembles that of the plant virus family Luteoviridae.
我们已经确定了在泰恩河畔纽卡斯尔分离出的人类1型星状病毒的完整基因组序列。该基因组长度为6813个核苷酸，包含三个连续的开放阅读框（ORF）。最靠近5'端的两个开放阅读框通过核糖体移码基序相连，并包含指示非结构病毒蛋白的序列基序：丝氨酸蛋白酶和RNA依赖性RNA聚合酶。一个核定位序列也位于此处。3'端的开放阅读框编码病毒粒子结构多肽，作为一种多蛋白前体。这种基因组结构类似于植物病毒黄症病毒科的结构。
