处于各种构象状态的谷氨酰胺结合蛋白晶体。

Crystals of glutamine-binding protein in various conformational states.

作者信息

Hsiao C D, Sun Y J, Rose J, Cottam P F, Ho C, Wang B C

机构信息

Department of Crystallography, University of Pittsburgh, PA 15260.

出版信息

J Mol Biol. 1994 Jul 1;240(1):87-91. doi: 10.1006/jmbi.1994.1420.

DOI:10.1006/jmbi.1994.1420

PMID:8021944

Abstract

Crystals of glutamine-binding protein (GlnBP) in various conformational states have been obtained. Crystals of the ligand-free "open" state (denoted form B) have unit cell dimensions a = 86.3 A, b = 86.3 A, c = 81.5 A, alpha = beta = gamma = 90 degrees and diffract to about 2.3 A resolution. An analysis of the intensity data using an Requiv plot indicates that the crystal system is orthorhombic, space group P2(1)2(1)2(1). Crystals of the ligand-bound "open" state (form B*) are obtained by soaking form B crystals with glutamine (Gln) and diffract to about 1.9 A. Crystals of the GlnBP-Gln complex in a ligand-bound "closed" state (form C) belong to space group P2(1)2(1)2(1) with a = 62.0 A, b = 65.7 A and c = 121.8 A and diffract to about 2.3 A. Crystals of a selenomethionyl GlnBP (form B') are isomorphous to form B crystals and diffract to about 2.1 A resolution.

摘要

已获得处于各种构象状态的谷氨酰胺结合蛋白（GlnBP）晶体。无配体“开放”状态（称为B型）的晶体，其晶胞参数为a = 86.3 Å，b = 86.3 Å，c = 81.5 Å，α = β = γ = 90°，衍射分辨率约为2.3 Å。使用Requiv图对强度数据进行分析表明，晶体系统为正交晶系，空间群为P2(1)2(1)2(1)。通过用谷氨酰胺（Gln）浸泡B型晶体可获得配体结合“开放”状态（B*型）的晶体，其衍射分辨率约为1.9 Å。配体结合“封闭”状态（C型）的GlnBP - Gln复合物晶体属于空间群P2(1)2(1)2(1)，a = 62.0 Å，b = 65.7 Å，c = 121.8 Å，衍射分辨率约为2.3 Å。硒代蛋氨酸GlnBP（B'型）晶体与B型晶体同晶型，衍射分辨率约为2.1 Å。