Ku W Y, Wang C S, Chen C Y, Chak K F, Safo M K, Yuan H S
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
Proteins. 1995 Dec;23(4):588-90. doi: 10.1002/prot.340230414.
The ImmE7 protein, which can bind specifically to the DNase colicin E7 and neutralize its bactericidal activity, has been purified and crystallized in two different crystal forms by vapor diffusion method. The orthorhombic crystals belong to space group I222 or I2(1)2(1)2(1) and have unit cell dimensions a = 75.1 A, b = 50.5 A, and c = 45.4 A. The second form is monoclinic space group P2(1) with cell dimensions a = 29.3 A, b = 102.7 A, c = 53.0 A, and beta = 91.5 degrees. The orthorhombic crystals diffract to 1.8 A resolution, and are suitable for high-resolution X-ray analysis.
ImmE7蛋白能特异性结合核酸酶大肠杆菌素E7并中和其杀菌活性，已通过气相扩散法纯化并结晶为两种不同的晶体形式。正交晶体属于空间群I222或I2(1)2(1)2(1)，晶胞参数为a = 75.1 Å，b = 50.5 Å，c = 45.4 Å。第二种形式是单斜空间群P2(1)，晶胞参数为a = 29.3 Å，b = 102.7 Å，c = 53.0 Å，β = 91.5°。正交晶体的衍射分辨率为1.8 Å，适用于高分辨率X射线分析。
