McAlarney T, Apostolski S, Lederman S, Latov N
Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
J Neurosci Res. 1994 Mar 1;37(4):453-60. doi: 10.1002/jnr.490370404.
We examined the binding of the gp120 envelope glycoprotein (gp120) of the human immunodeficiency virus (HIV-1) to sulfatide (GalS), galactocerebroside (GalC), and GM1-ganglioside (GM1). The gp120 glycoprotein bound to GalS but not to GalC or GM1 by enzyme-linked immunosorbent assay (ELISA) and by an immunospot assay on nitrocellulose paper. However, it bound to all three glycolipids by an immunospot assay on thin layer chromatography (TLC) plates. In studies to determine whether GalS could be a receptor for gp120 on the surface of cells, gp120 bound to GalS incorporated into the plasma membrane of lymphoid cells as determined by cytofluorometric analysis and immunofluorescence microscopy. These studies indicate that GalS may function as a receptor for gp120 and HIV-1.
我们检测了人类免疫缺陷病毒1型（HIV-1）的包膜糖蛋白gp120与硫苷脂（GalS）、半乳糖脑苷脂（GalC）和GM1神经节苷脂（GM1）的结合情况。通过酶联免疫吸附测定（ELISA）以及在硝酸纤维素纸上进行的免疫斑点测定，gp120糖蛋白与GalS结合，但不与GalC或GM1结合。然而，通过在薄层色谱（TLC）板上进行的免疫斑点测定，它与所有三种糖脂都结合。在确定GalS是否可能是细胞表面gp120的受体的研究中，通过细胞荧光分析和免疫荧光显微镜检查确定，gp120与掺入淋巴细胞质膜的GalS结合。这些研究表明，GalS可能作为gp120和HIV-1的受体发挥作用。
