Frey P, Lutz N, Berger D, Herzog B
Department of Pediatric Surgery, CHUV, Lausanne, Switzerland.
J Urol. 1994 Aug;152(2 Pt 2):632-5. doi: 10.1016/s0022-5347(17)32669-1.
Endoscopic subureteral collagen injection has become an accepted means for the treatment of vesicoureteral reflux in children. The aim of this study was to evaluate the histological behavior of glutaraldehyde cross-linked bovine collagen implants. The specimens were harvested from 29 patients who underwent reimplant surgery 2 to 30 months (mean 9.5) after unsuccessful subureteral injection therapy. In addition to routine hematoxylin and eosin staining, a new staining method (solophenyl red 3BL) able to demonstrate selectively neoformation of types I and III human collagen, was applied. Invasion of host fibroblasts into the bovine implant and the formation of endogenous types I and III collagen were demonstrated in all 29 cases. Adverse histological reactions were rare and, if present, they were predominantly of an inflammatory nature.
内镜下输尿管下胶原蛋白注射已成为治疗儿童膀胱输尿管反流的一种公认方法。本研究的目的是评估戊二醛交联牛胶原蛋白植入物的组织学行为。标本取自29例患者，这些患者在输尿管下注射治疗失败后2至30个月（平均9.5个月）接受了再植手术。除常规苏木精和伊红染色外，还应用了一种能够选择性显示I型和III型人胶原蛋白新形成的新染色方法（单苯基红3BL）。在所有29例病例中均证实宿主成纤维细胞侵入牛植入物并形成内源性I型和III型胶原蛋白。不良组织学反应很少见，如有发生，主要为炎症性质。
