Ellozy A R, Wang R H, Dillon J
College at Lincoln Center, Fordham University, New York, NY 10023.
Photochem Photobiol. 1994 Apr;59(4):474-8. doi: 10.1111/j.1751-1097.1994.tb05067.x.
Intact young human, baboon and rhesus monkey lenses were subjected to near-UV irradiation under identical conditions and fluorophore buildup was continuously monitored for several hours. The compositional changes occurring in the lenses were monitored by analyzing the ethanol extracts of the irradiated and control lenses using high-performance liquid chromatography and thin-layer chromatography (TLC). The chromatograms of the supernatant detected at 365 nm as well as the TLC scans showed the presence of 3-hydroxykynurenine glucoside (3-HKG) and two other kynurenine-type compounds. The 3-HKG and one of the compounds were found in all three species, while the remaining one was structurally different in the lower primates. A loss of 3-HKG as a result of irradiation was apparent in all the lenses and correlated with the buildup of the blue fluorophore, suggesting that the latter may be a photoproduct(s) of 3-HKG. The kinetic analysis of baboon and human lenses showed a slowdown in the fluorophore buildup as irradiation times increased. This was probably due to the competitive absorptions of 3-HKG and other chromophores present. Rhesus monkey lenses did not exhibit this slowdown.
完整的年轻人类、狒狒和恒河猴晶状体在相同条件下接受近紫外线照射，并连续数小时监测荧光团的积累情况。通过使用高效液相色谱法和薄层色谱法（TLC）分析照射过的晶状体和对照晶状体的乙醇提取物，监测晶状体中发生的成分变化。在365nm处检测到的上清液色谱图以及TLC扫描显示存在3-羟基犬尿氨酸葡萄糖苷（3-HKG）和另外两种犬尿氨酸类化合物。在所有三个物种中都发现了3-HKG和其中一种化合物，而在低等灵长类动物中，其余一种在结构上有所不同。在所有晶状体中，照射导致3-HKG减少，并且与蓝色荧光团的积累相关，这表明后者可能是3-HKG的光产物。对狒狒和人类晶状体的动力学分析表明，随着照射时间的增加，荧光团积累速度减慢。这可能是由于存在的3-HKG和其他发色团的竞争性吸收。恒河猴晶状体没有表现出这种减慢。
