Martin Sanchez J, Morillas Marquez F, Sanchiz Marin M C, Acedo Sanchez C
Departamento de Parasitologia, Facultad de Farmacia, Universidad de Granada, Spain.
Am J Trop Med Hyg. 1994 Jun;50(6):758-62. doi: 10.4269/ajtmh.1994.50.758.
Sixty-four strains of Leishmania of canine origin, 61 visceral and three cutaneous, were isoenzymatically examined. These were collected from 23 sites in the Granada region in southern Spain. Starch gel was used in electrophoresis and a total of 15 enzymes were studied. All of the visceral strains and two of the cutaneous ones were identified as L. infantum zymodeme GR-1 (= MON-1). The third cutaneous strain was of a different zymodeme, belonging to the same complex but differing in the glucose-6-phosphate dehydrogenase (= 105) enzyme. This zymodeme has been named GR-16 and is equivalent to L. infantum MON-105; it has not been previously reported in dogs or any other animal reservoir.
对64株犬源利什曼原虫进行了同工酶检测，其中61株为内脏型，3株为皮肤型。这些菌株采自西班牙南部格拉纳达地区的23个地点。采用淀粉凝胶进行电泳，并对总共15种酶进行了研究。所有内脏型菌株和2株皮肤型菌株被鉴定为婴儿利什曼原虫酶带型GR-1（=MON-1）。第三株皮肤型菌株属于不同的酶带型，属于同一复合体，但在葡萄糖-6-磷酸脱氢酶（=105）酶方面存在差异。这种酶带型被命名为GR-16，等同于婴儿利什曼原虫MON-105；此前在犬类或任何其他动物宿主中均未报道过。
