Martín-Sánchez J, Guilvard E, Acedo-Sánchez C, Wolf-Echeverri M, Sanchiz-Marín M C, Morillas-Márquez F
Department of Parasitology, Faculty of Pharmacy, Granada, Spain.
Int J Parasitol. 1994 May;24(3):405-8. doi: 10.1016/0020-7519(94)90089-2.
This study presents the results of the isoenzymatic characterization of 21 strains of Leishmania of sandfly (P. perniciosus) origin from the Torvizcón area. It forms an integral part of a larger eco-epidemiological study of the Alpujarras (Granada province, Southern Spain). The strains analysed were shown to belong to the L. infantum complex based on the results of 15 enzymes. The electrophoretic profiles for the enzymes MDH, G6PD and NP1 have permitted the identification of four zymodemes: GR-1 (5 strains), GR-2 (2 strains), GR-3 (13 strains) and GR-7 (1 strain); only one of these zymodemes, GR-1, was found in the Torvizcón area in the vertebrate host (man and dog). This is the first time zymodeme GR-7 has been described.
本研究展示了来自托维斯科恩地区、起源于白蛉（P. perniciosus）的21株利什曼原虫同工酶特征分析的结果。它是阿尔普哈拉斯地区（西班牙南部格拉纳达省）一项更大规模生态流行病学研究的组成部分。根据15种酶的检测结果，所分析的菌株被证明属于婴儿利什曼原虫复合体。MDH、G6PD和NP1这几种酶的电泳图谱已鉴定出四种酶型：GR - 1（5株）、GR - 2（2株）、GR - 3（13株）和GR - 7（1株）；在托维斯科恩地区的脊椎动物宿主（人和狗）中仅发现了其中一种酶型GR - 1。这是首次描述酶型GR - 7。
