中耳炎患儿对卡他莫拉菌的免疫反应：用抗原包被的乳胶珠进行调理吞噬作用。

Immune response to Moraxella catarrhalis in children with otitis media: opsonophagocytosis with antigen-coated latex beads.

作者信息

Faden H, Hong J J, Pahade N

机构信息

Department of Pediatrics, State University of New York, Buffalo School of Medicine.

出版信息

Ann Otol Rhinol Laryngol. 1994 Jul;103(7):522-4. doi: 10.1177/000348949410300704.

DOI:10.1177/000348949410300704

PMID:8024214

Abstract

Opsonic antibody activity against Moraxella catarrhalis was determined in sera from children with otitis media. The antibody was determined with a new assay utilizing outer membrane antigen-coated latex beads. Antigen-coated beads opsonized in heat-inactivated pooled human serum phagocytosed 47.5 +/- 36.1 beads per 100 neutrophils compared to 15.6 +/- 10.2 beads per 100 neutrophils opsonized in hypogammaglobulinemic serum (p < .025). Antigen-coated beads opsonized in homologous sera from 11 children with M. catarrhalis otitis media demonstrated increased opsonic activity in convalescent sera (34.6 +/- 27.1) compared to acute sera (15.5 +/- 6.7; p < .05). These data suggest that infection with M catarrhalis is associated with the development of opsonic antibody.

摘要

在患有中耳炎的儿童血清中测定了抗卡他莫拉菌的调理素抗体活性。该抗体通过一种利用外膜抗原包被乳胶珠的新检测方法来测定。在热灭活的混合人血清中调理的抗原包被珠，每100个中性粒细胞吞噬47.5±36.1个珠子，而在低丙种球蛋白血症血清中调理的每100个中性粒细胞吞噬15.6±10.2个珠子（p<0.025）。在11名患有卡他莫拉菌中耳炎的儿童的同源血清中调理的抗原包被珠，与急性期血清（15.5±6.7）相比，恢复期血清（34.6±27.1）显示出增强的调理活性（p<0.05）。这些数据表明，卡他莫拉菌感染与调理素抗体的产生有关。