Bitton G, Jung K, Koopman B
Department of Environmental Engineering Sciences, University of Florida, Gainesville 32611.
Arch Environ Contam Toxicol. 1994 Jul;27(1):25-8. doi: 10.1007/BF00203883.
A rapid, quantitative microbial assay, which is specific for heavy metal toxicity, has been developed. The assay (MetPLATE) is in a 96-well microtitration plate format and is suitable for determining toxicity characteristics such as median inhibitory concentrations. The sensitivity of MetPLATE to heavy metals [Cu, Zn, Cd, Pb, Hg, Cr(III)] was generally higher than Microtox and was of the same order as or better than Daphnia and fish bioassay. MetPLATE was insensitive to organic compounds at concentrations higher than those found in the environment. Six out of 10 industrial wastewaters or process waters surveyed were toxic. Heavy metal analysis of these waters confirmed the presence of heavy metals in the toxic samples. MetPLATE can be run concurrently with other assays for general toxicity to help determine the nature of chemicals causing toxicity.
已开发出一种针对重金属毒性的快速定量微生物检测方法。该检测方法（MetPLATE）采用96孔微量滴定板形式，适用于确定诸如半数抑制浓度等毒性特征。MetPLATE对重金属[铜、锌、镉、铅、汞、铬（III）]的灵敏度通常高于Microtox，与水蚤和鱼类生物检测的灵敏度相当或更好。MetPLATE对浓度高于环境中发现的有机化合物不敏感。在调查的10种工业废水或工艺用水中，有6种具有毒性。对这些水的重金属分析证实了有毒样品中存在重金属。MetPLATE可与其他一般毒性检测方法同时进行，以帮助确定导致毒性的化学物质的性质。
