Nakagawa M, Kukita T, Nakasima A, Kurisu K
Department of Orthodontics, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Arch Oral Biol. 1994 Apr;39(4):289-94. doi: 10.1016/0003-9969(94)90119-8.
The in situ hybridization technique used digoxigenin-labelled oligodeoxynucleotide. In untreated molars, cells expressing a positive signal for type I collagen mRNA were distributed uniformly in the periodontal ligament space. After experimental tooth movement, the density of cells expressing a positive signal appeared to be much greater in the tension side than the pressure side. In both sides the distribution of the positively hybridizing cells was uniform along the principal fibres of the ligament. This characteristic distribution appeared at 12 h after the initiation of tooth movement, reached a maximum at 1-3 days, and persisted for about 14 days during the treatment. These results indicate that the remodelling of collagen fibres in periodontal ligament occurs in an orderly manner throughout the principal fibres, mainly on the tension side, and that the recovery of gene expression for type I collagen occurs within the first 14 days in response to experimental tooth movement.
原位杂交技术使用地高辛标记的寡脱氧核苷酸。在未处理的磨牙中，I型胶原mRNA表达阳性信号的细胞均匀分布于牙周膜间隙。实验性牙齿移动后，表达阳性信号的细胞密度在张力侧似乎比压力侧大得多。在两侧，阳性杂交细胞沿韧带主纤维的分布是均匀的。这种特征性分布在牙齿移动开始后12小时出现，在1 - 3天达到最大值，并在治疗期间持续约14天。这些结果表明，牙周膜中胶原纤维的重塑在整个主纤维上有序发生，主要在张力侧，并且I型胶原基因表达的恢复在实验性牙齿移动后的前14天内发生。
