Ishiguro K, Yamashita K, Nakagaki H, Iwata K, Hayakawa T
Department of Preventive Dentistry and Dental Public Health, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Arch Oral Biol. 1994 Apr;39(4):345-9. doi: 10.1016/0003-9969(94)90126-0.
An inhibitory activity toward matrix metalloproteinases such as interstitial collagenase, 72-kDa gelatinase/type IV collagenase, and stromelysin-1 was detected in an EDTA extract of pulverized roots of human teeth, and identified as TIMP-1 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting. Distribution of TIMP-1 in human cementum and dentine was investigated by a sandwich enzyme immunoassay in combination with an abrasive microsampling technique. TIMP-1 could not be detected in cementum from some teeth but in others decreased from a fairly low value at the surface towards the cementodentinal junction. TIMP-1 concentrations in the dentine increased consistently from the cementodentinal junction toward the predentine. The average TIMP-1 concentration in the dentine (54.1 +/- 18.5 pg/mg +/- SE) was significantly (P < 0.05) higher than that (9.6 +/- 6.0 pg/mg +/- SE) in the cementum.
在人牙齿磨碎根的乙二胺四乙酸（EDTA）提取物中检测到对基质金属蛋白酶（如间质胶原酶、72 kDa 明胶酶/IV 型胶原酶和基质溶解素-1）的抑制活性，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹法鉴定为金属蛋白酶组织抑制因子-1（TIMP-1）。采用夹心酶免疫测定法结合研磨微取样技术研究了 TIMP-1 在人牙骨质和牙本质中的分布。在一些牙齿的牙骨质中未检测到 TIMP-1，但在其他牙齿中，其含量从表面相当低的值向牙骨质牙本质界逐渐降低。牙本质中的 TIMP-1 浓度从牙骨质牙本质界向前期牙本质持续增加。牙本质中 TIMP-1 的平均浓度（54.1±18.5 pg/mg±标准误）显著高于牙骨质中的浓度（9.6±6.0 pg/mg±标准误）（P<0.05）。
