Paddock S W
Howard Hughes Medical Institute, Laboratory of Molecular Biology, University of Wisconsin, Madison 53706.
Bioessays. 1994 May;16(5):357-65. doi: 10.1002/bies.950160511.
The laser scanning confocal microscope (LSCM) is now established as an invaluable tool in developmental biology for improved light microscope imaging of fluorescently labelled eggs, embryos and developing tissues. The universal application of the LSCM in biomedical research has stimulated improvements to the microscopes themselves and the synthesis of novel probes for imaging biological structures and physiological processes. Moreover the ability of the LSCM to produce an optical series in perfect register has made computer 3-D reconstruction and analysis of light microscope images a practical option.
激光扫描共聚焦显微镜（LSCM）现已成为发育生物学中一种极具价值的工具，可用于对荧光标记的卵子、胚胎和发育组织进行改进的光学显微镜成像。LSCM在生物医学研究中的广泛应用推动了显微镜自身的改进以及用于生物结构和生理过程成像的新型探针的合成。此外，LSCM能够生成精确对齐的光学系列，这使得计算机对光学显微镜图像进行三维重建和分析成为一种可行的选择。
