异常或停滞的人类植入前胚胎的激光扫描共聚焦成像
Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos.
作者信息
Levy R, Benchaib M, Cordonier H, Souchier C, Guerin J F
机构信息
Laboratoire de Biologie de la Reproduction et du Développement, Hôpital Edouard Herriot, Lyon, France.
出版信息
J Assist Reprod Genet. 1998 Sep;15(8):485-95. doi: 10.1023/a:1022582404181.
PURPOSE
The improved resolution and optical sectioning of a confocal microscope make it an ideal instrument for extracting three-dimensional information, especially from extended biological specimens such as human embryos. The staining of actin together with chromatin allowed us to specify the architecture of the embryo and the appearance of the nucleus.
METHODS
F-Actin and chromatin distributions were visualized using laser scanning confocal microscopy in "fresh" and "cryopreserved" human preimplantation embryos obtained by in vitro fertilization.
RESULTS
The current study revealed a high rate of multinucleation in arrested or poor-quality embryos (89%, in grade IV embryos).
CONCLUSIONS
Confocal microscopy revealed high levels of multinucleated blastomeres, suggesting that the probable cause of arrested development in these embryos was due to multinucleation of blastomeres.
目的
共聚焦显微镜分辨率的提高和光学切片功能使其成为提取三维信息的理想仪器，特别是从诸如人类胚胎等扩展的生物标本中获取信息。肌动蛋白与染色质一起染色使我们能够明确胚胎的结构和细胞核的外观。
方法
使用激光扫描共聚焦显微镜观察通过体外受精获得的“新鲜”和“冷冻保存”的人类植入前胚胎中F-肌动蛋白和染色质的分布。
结果
当前研究揭示了停滞或质量较差的胚胎中多核化发生率很高（IV级胚胎中为89%）。
结论
共聚焦显微镜显示出高水平的多核卵裂球，表明这些胚胎发育停滞的可能原因是卵裂球的多核化。
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