Barch D H, Rundhaugen L M, Thomas P E, Kardos P, Pillay N S
Department of Medicine, Lakeside Veterans Affairs Medical Center, Northwestern University Medical School, Chicago, Illinois.
Biochem Biophys Res Commun. 1994 Jun 30;201(3):1477-82. doi: 10.1006/bbrc.1994.1870.
The anticarcinogen ellagic acid significantly reduces the incidence of polycyclic aromatic hydrocarbon induced carcinomas, in association with significant reductions in benzo[a]pyrene hydroxylase activity and CO reducible hepatic cytochrome P450. This suggested that ellagic acid reduced hepatic concentrations of CYP1A1, but these changes occurred without any alteration in CYP1A1 mRNA or in immunoreactive CYP1A1. Dietary ellagic acid results in muMolar concentrations of ellagic acid in hepatic tissue. Addition of similar concentrations of ellagic acid in vitro directly inhibited the measurement of benzo[a]pyrene hydroxylase activity and CO reducible cytochrome P450. Thus dietary ellagic acid does not alter the hepatic concentration of CYP1A1, but the dietary ellagic acid remaining in hepatic tissue appears to directly inhibit the measurement of benzo[a]pyrene hydroxylase activity and CO reducible cytochrome P450.
抗癌剂鞣花酸可显著降低多环芳烃诱导的癌症发病率，同时显著降低苯并[a]芘羟化酶活性和一氧化碳可还原的肝细胞色素P450。这表明鞣花酸降低了肝脏中CYP1A1的浓度，但这些变化并未伴随CYP1A1 mRNA或免疫反应性CYP1A1的任何改变。膳食中的鞣花酸会在肝组织中产生微摩尔浓度的鞣花酸。在体外添加相似浓度的鞣花酸可直接抑制苯并[a]芘羟化酶活性和一氧化碳可还原细胞色素P450的测定。因此，膳食中的鞣花酸不会改变肝脏中CYP1A1的浓度，但残留在肝组织中的膳食鞣花酸似乎会直接抑制苯并[a]芘羟化酶活性和一氧化碳可还原细胞色素P450的测定。
