Rönnelid J, Lysholm J, Engström-Laurent A, Klareskog L, Heyman B
University Hospital, Uppsala, Sweden.
Arthritis Rheum. 1994 Jul;37(7):1023-9. doi: 10.1002/art.1780370707.
To investigate the production of type II collagen (CII) antibodies in the synovial fluid (SF) of rheumatoid arthritis (RA) patients, and to examine the HLA dependence of this local production.
The ELISPOT method was used for enumerating anti-CII-reactive cells. Serologic tissue typing was performed.
Anti-CII-reactive cells were found in the SF of 16 of 31 patients, but not in any of the peripheral blood samples obtained in parallel. SF anti-CII antibody production showed no correlation with clinical parameters, but its frequency increased significantly with age. The IgG anti-CII response occurred exclusively in patients who were positive for HLA-DR4 and was significantly associated with DR4.
Anti-CII production may be important in local immune complex formation. The indirect demonstration of a DR4-restricted T cell response to CII is an indication of a pathogenetic role of collagen autoimmunity in RA.
研究类风湿关节炎（RA）患者滑液（SF）中Ⅱ型胶原（CII）抗体的产生，并检测这种局部产生的HLA依赖性。
采用ELISPOT法计数抗CII反应性细胞。进行血清学组织分型。
31例患者中有16例的滑液中发现抗CII反应性细胞，但同期采集的外周血样本中均未发现。滑液抗CII抗体产生与临床参数无相关性，但其频率随年龄显著增加。IgG抗CII反应仅发生在HLA-DR4阳性患者中，且与DR4显著相关。
抗CII产生可能在局部免疫复合物形成中起重要作用。对CII的DR4限制性T细胞反应的间接证明表明胶原自身免疫在RA发病机制中起作用。
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