Mullen M A, Wang H, Wilcox K, Herman T
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226.
DNA Cell Biol. 1994 May;13(5):521-30. doi: 10.1089/dna.1994.13.521.
The structure of the Max:DNA complex was investigated by cross-linking Max to a series of photoactive oligonucleotides. A single photoactive aryl azide was introduced into oligonucleotides at defined positions downstream from the specific CACGTG binding site. Purified Max homodimers bound to and were cross-linked to oligonucleotides containing a photoactive group either 2, 5, 8, or 11 bp downstream from the binding site. Further analysis revealed that an amino-terminal chymotryptic peptide of Max was cross-linked to the oligonucleotide containing a photoactive probe 11 bp downstream from the specific binding site. This result is consistent with the recent crystal structure of the Max:DNA complex (Ferré-D'Amaré et al., 1993) and further suggests that amino acid residues near the amino-terminus of Max are in close proximity to a region of DNA that is separated from the core binding site by one turn of the double helix.
通过将Max与一系列光活性寡核苷酸交联，研究了Max:DNA复合物的结构。在特定的CACGTG结合位点下游的特定位置，将单个光活性芳基叠氮化物引入寡核苷酸中。纯化的Max同二聚体与结合位点下游2、5、8或11个碱基对处含有光活性基团的寡核苷酸结合并交联。进一步分析表明，Max的氨基末端胰凝乳蛋白酶肽与特定结合位点下游11个碱基对处含有光活性探针的寡核苷酸交联。这一结果与最近的Max:DNA复合物晶体结构（Ferré-D'Amaré等人，1993年）一致，并进一步表明Max氨基末端附近的氨基酸残基与DNA的一个区域紧密相邻，该区域与核心结合位点相隔双螺旋的一圈。
