Jin Ya'nan, Zhai Shanshan, Wang Wenjia, Ding Xihan, Guo Zhifu, Bai Liping, Wang Shu
1College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, 110866 Liaoning China.
2College of Agronomy, Shenyang Agricultural University, Shenyang, 110866 Liaoning China.
Physiol Mol Biol Plants. 2018 Mar;24(2):211-229. doi: 10.1007/s12298-017-0495-y. Epub 2017 Dec 22.
Adverse environmental conditions limit various aspects of plant growth, productivity, and ecological distribution. To get more insights into the signaling pathways under low temperature, we identified 10 C-repeat binding factors (), 9 inducer of CBF expression () and 10 cold-responsive () genes from - composite group under cold stress. Conserved amino acids analysis revealed that all CBF, ICE, COR contained specific and typical functional domains. Phylogenetic analysis of CBF proteins from showed that these CBF homologs were divided into 11 groups. CBFs from were found in every group, which shows that these CBFs generated prior to the divergence of the subfamilies of . The evolutionary relationship among the ICE and COR proteins in were divided into four groups with high multispecies specificity, respectively. Moreover, expression analysis revealed that mRNA accumulation was altered by cold treatment and the genes of three types involved in the ICE-CBF-COR signaling pathway were induced by cold stress. Together, the results make , , genes family in more abundant, and provide a starting point for future studies on transcriptional regulatory network for improvement of chilling tolerance in crop.
不利的环境条件限制了植物生长、生产力和生态分布的各个方面。为了更深入了解低温下的信号通路，我们从冷胁迫下的拟南芥复合组中鉴定出10个C-重复结合因子（CBF）、9个CBF表达诱导因子（ICE）和10个冷响应（COR）基因。保守氨基酸分析表明，所有CBF、ICE、COR都含有特定且典型的功能结构域。对拟南芥CBF蛋白的系统发育分析表明，这些CBF同源物被分为11组。拟南芥中的CBF存在于每组中，这表明这些CBF在拟南芥亚科分化之前就已产生。拟南芥中ICE和COR蛋白之间的进化关系分别分为具有高多物种特异性的四组。此外，表达分析表明，冷处理会改变mRNA积累，并且参与ICE-CBF-COR信号通路的三种类型的基因在冷胁迫下被诱导。总之，这些结果使拟南芥中的CBF、ICE、COR基因家族更加丰富，并为未来研究提高作物耐寒性的转录调控网络提供了一个起点。
