Ross U H, Rogowski M, Reiss G, Gloddek B
Department of Oto-Rhino-Laryngology, University of Freiburg, Germany.
Eur Arch Otorhinolaryngol. 1994;251(2):80-3. doi: 10.1007/BF00179897.
For the evaluation of functional inner ear lesions caused by an autoimmune-induced labyrinthitis, transiently evoked otoacoustic emissions (TEOAEs) were detected in guinea pigs before and after transfer of sensitized lymphocytes from animals suffering from a labyrinthitis induced by the foreign protein keyhole limpet hemocyanin (KLH). Initially TEOAEs were detectable from 47 of 62 ears (76%) in healthy guinea pigs. These animals then were used as recipients for sensitized lymphocytes from donors exclusively. Three months after cell transfer the incidence of TEOAEs was reduced to 48% in animals receiving lymphocytes from donors sensitized with KLH intradermally and intracochlearly. In recipients of lymphocytes from donors sensitized only intradermally and in untreated animals no significant alteration of the TEOAE incidence was found. These findings showed strong correlation with those from scanning electron microscopy of the organ of Corti, indicating that the measurement of TEOAE is a useful, time-saving tool for the detection of cochlear dysfunction caused by sensitized lymphocytes in experimental animal. The present findings also show that the migration of sensitized lymphocytes actually leads to functional lesions in the cochlea.
为了评估自身免疫性诱导的迷路炎所导致的功能性内耳损伤，在将患有由外源性蛋白质钥孔戚血蓝蛋白（KLH）诱导的迷路炎的动物的致敏淋巴细胞进行转移之前和之后，对豚鼠检测了瞬态诱发耳声发射（TEOAEs）。最初，在健康豚鼠的62只耳朵中有47只（76%）可检测到TEOAEs。然后这些动物仅用作来自供体的致敏淋巴细胞的受体。细胞转移三个月后，在接受经皮内和耳蜗内用KLH致敏的供体的淋巴细胞的动物中，TEOAEs的发生率降至48%。在接受仅经皮内致敏的供体的淋巴细胞的受体以及未治疗的动物中，未发现TEOAEs发生率有显著改变。这些发现与来自柯蒂氏器扫描电子显微镜的发现密切相关，表明TEOAEs的测量是检测实验动物中由致敏淋巴细胞引起的耳蜗功能障碍的一种有用且省时的工具。目前的发现还表明，致敏淋巴细胞的迁移实际上会导致耳蜗中的功能性损伤。
