Chen M C, Harris J P, Keithley E M
Division of Otolaryngology-Head and Neck Surgery, University of California, San Diego, and the Research Services of Veterans Affairs, La Jolla 92093, USA.
Laryngoscope. 1998 May;108(5):651-6. doi: 10.1097/00005537-199805000-00005.
Inflammatory reactions within the cochlea lead to the formation of fibrotic tissue and bone. To determine which cells are involved in the proliferation of the inflammatory response within the cochlea, sterile labyrinthitis was created by inoculating keyhole limpet hemocyanin (KLH) into the scala tympani of systemically sensitized animals. Cellular proliferation was assessed immunohistochemically using the monoclonal antibody Ki-67. Proliferating cells were identified among inflammatory cells and fibroblasts within the matrix, as well as in endosteal cells lining the scala tympani. Inflammatory and potential osteoprogenitor cells were labeled as late as 6 weeks after inoculation, suggesting the absence of a strong immunosuppressive mechanism. Endosteal cells may proliferate and secrete the extracellular matrix used by the inflammatory cells to move within the cochlear scalae. They may also participate in the ossification of the inflammatory matrix.
耳蜗内的炎症反应会导致纤维化组织和骨的形成。为了确定哪些细胞参与耳蜗内炎症反应的增殖，通过将钥孔戚血蓝蛋白（KLH）接种到全身致敏动物的鼓阶中，制造了无菌性迷路炎。使用单克隆抗体Ki-67通过免疫组织化学方法评估细胞增殖。在基质内的炎症细胞和成纤维细胞以及鼓阶内衬的骨内膜细胞中鉴定出增殖细胞。接种后长达6周，炎症细胞和潜在的骨祖细胞仍被标记，这表明缺乏强大的免疫抑制机制。骨内膜细胞可能会增殖并分泌炎症细胞在耳蜗管内移动所使用的细胞外基质。它们也可能参与炎症基质的骨化。
