氯化镁浓度对随机扩增多态性DNA的影响。
Effect of concentration of MgCl2 on random-amplified DNA polymorphism.
作者信息
Park Y H, Kohel R J
机构信息
USDA-ARS Southern Crops Research Laboratory, College Station, TX.
出版信息
Biotechniques. 1994 Apr;16(4):652-6.
The influence of MgCl2 concentration on products in PCR to generate random-amplified polymorphic DNA (RAPD) was evaluated between two highly polymorphic cotton lines. Varying the concentration of MgCl2 (1.5 to 6.0 mM) in the PCR mixtures resulted in both quantitative differences in RAPD PCR-derived DNA bands and qualitative changes in the DNA band patterns. The optimum concentration of MgCl2 was within the range of 1.5 to 4.5 mM in the PCR mixtures for 154 of 200 random primers tested. The optimum concentration of MgCl2 for each primer was independent of the GC/AT ratio of the primer and the number of DNA band(s) amplified.
在两个高度多态的棉花品系之间评估了MgCl2浓度对聚合酶链反应（PCR）中用于产生随机扩增多态性DNA（RAPD）产物的影响。在PCR混合物中改变MgCl2的浓度（1.5至6.0 mM），导致RAPD PCR衍生的DNA条带出现数量差异以及DNA条带模式的质量变化。对于所测试的200种随机引物中的154种，PCR混合物中MgCl2的最佳浓度在1.5至4.5 mM范围内。每种引物的MgCl2最佳浓度与引物的GC/AT比率以及扩增的DNA条带数量无关。
Zotero 插件