Venugopal G, Mohapatra S, Salo D, Mohapatra S
Department of Immunology, University of Manitoba, Winnipeg, Canada.
Biochem Biophys Res Commun. 1993 Dec 30;197(3):1382-7. doi: 10.1006/bbrc.1993.2630.
In order to investigate the possible mechanism underlying the random amplified polymorphic DNA (RAPD) fingerprinting, we examined the origin and the nucleotide sequences of RAPD bands. Our data suggest that a number of sites in the genome are flanked by perfect or imperfect invert repeats, which permit multiple mismatch-annealing to occur between the single primer and the template DNA and eventually lead to the exponential amplification of the encompassing DNA segments.
为了研究随机扩增多态性DNA（RAPD）指纹图谱潜在的可能机制，我们检测了RAPD条带的起源和核苷酸序列。我们的数据表明，基因组中的许多位点两侧是完美或不完美的反向重复序列，这使得单引物与模板DNA之间能够发生多次错配退火，最终导致所包含的DNA片段呈指数扩增。
