Freedman A S, Saporito L, Rhynhart K, Morimoto C, Nadler L M
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Leuk Lymphoma. 1994 Mar;13(1-2):47-52. doi: 10.3109/10428199409051651.
Follicular non-Hodgkin's lymphomas (NHL) generally present as disseminated diseases with infiltration of lymphoid organs, bone marrow (BM), as well as peripheral blood (PB). These lymphoma cells may recapitulate the behavior of normal germinal center (GC) B cells, some of which remain in follicles and others which have the capacity to migrate. Normal activated B cells and follicular lymphoma cells bind to GCs in vitro and this interaction is mediated by VLA-4 on the lymphoid cell and VCAM-1 on follicular dendritic cells. Since the disseminated nature of follicular NHLs may be related to the ability of PB and BM cells to recirculate through lymphoid tissues, we examined the adhesive characteristics of follicular lymphoma cells isolated from these site. Cells from 10 of 14 cases of follicular NHL involving PB or BM bound to normal GCs. Neoplastic GCs could similarly support the binding of PB or BM derived follicular NHL cells. This interaction was inhibited by monoclonal antibodies directed against VLA-4 and VCAM-1. These studies may provide insight into the clinical behavior of these diseases. More importantly the homing and adhesion of lymphoma cells is likely to be relevant to the use of PB and BM as a source of hematopoietic stem cells following high dose ablative therapy.
滤泡性非霍奇金淋巴瘤（NHL）通常表现为播散性疾病，伴有淋巴器官、骨髓（BM）以及外周血（PB）浸润。这些淋巴瘤细胞可能重现正常生发中心（GC）B细胞的行为，其中一些细胞留在滤泡中，另一些具有迁移能力。正常活化B细胞和滤泡性淋巴瘤细胞在体外与GC结合，这种相互作用由淋巴样细胞上的VLA-4和滤泡树突状细胞上的VCAM-1介导。由于滤泡性NHL的播散性质可能与PB和BM细胞通过淋巴组织再循环的能力有关，我们研究了从这些部位分离的滤泡性淋巴瘤细胞的黏附特性。14例累及PB或BM的滤泡性NHL中，有10例的细胞与正常GC结合。肿瘤性GC同样可以支持PB或BM来源的滤泡性NHL细胞的结合。这种相互作用被针对VLA-4和VCAM-1的单克隆抗体抑制。这些研究可能有助于深入了解这些疾病的临床行为。更重要的是，淋巴瘤细胞的归巢和黏附可能与高剂量消融治疗后将PB和BM用作造血干细胞来源有关。
