Ali A
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Lupus. 1994 Feb;3(1):43-6. doi: 10.1177/096120339400300109.
The present study was designed to study the possible role of the female sex hormone, estradiol in conjugation with DNA, in the pathogenesis of systemic lupus erythematosus (SLE). Calf thymus DNA fragments (average size 200 bp) were covalently linked to estradiol-albumin (E2-BSA) by treatment with glutaraldehyde. The conjugate was separated from free DNA by gel exclusion chromatography. It was characterized by UV spectroscopy and thermal denaturation studies. The conjugate (E2-BSA-DNA) induced high titer antibodies which were highly specific towards their immunogen. These antibodies also recognize estradiol-albumin and beta-estradiol but not native DNA (B-form), brominated DNA (Z-form) or poly(rG).poly(dC) (A-form). The E2-BSA-DNA conjugate showed significant binding with SLE sera known to have a high level of anti-native DNA antibodies. The results indicated the recognition of unique conformation of estradiol-BSA-DNA conjugate by naturally occurring SLE anti-DNA autoantibodies.
本研究旨在探讨雌性激素雌二醇与DNA结合在系统性红斑狼疮（SLE）发病机制中的可能作用。通过戊二醛处理，将小牛胸腺DNA片段（平均大小200 bp）与雌二醇 - 白蛋白（E2 - BSA）共价连接。通过凝胶排阻色谱法将缀合物与游离DNA分离。通过紫外光谱和热变性研究对其进行表征。缀合物（E2 - BSA - DNA）诱导产生高滴度抗体，这些抗体对其免疫原具有高度特异性。这些抗体也识别雌二醇 - 白蛋白和β - 雌二醇，但不识别天然DNA（B型）、溴化DNA（Z型）或聚（rG）·聚（dC）（A型）。E2 - BSA - DNA缀合物与已知具有高水平抗天然DNA抗体的SLE血清显示出显著结合。结果表明，天然存在的SLE抗DNA自身抗体能够识别雌二醇 - BSA - DNA缀合物的独特构象。
